PentaBase is manufacturing oligonucleotides based on standard and proprietary DNA technologies
We are currently supplying our customers with oligonucleotides for SARS-CoV-2 PCR assays based on our proprietary INA® technologies including SuPrimers™ and HydrolEasy™ probes.
Standard DNA primers
Post PCR melt analysis of SARS-CoV-2 PCR amplicons generated using standard DNA primers (left) and SuPrimers™ (right).
SuPrimers™ are PCR primers modified with our proprietary technologies. The benefits of replacing primers based on standard DNA with SuPrimers™ include increased signal-to-noise ratio as well as increased sensitivity and specificity towards their target sequences
Real-time PCR amplification plots of SARS-CoV-2 PCR assay with standard DNA primers (red lines), one SuPrimer™ and one standard primer (blue lines), and two SuPrimers™ (green lines).
HydrolEasy™ probes are dual-labeled hydrolysis probes based on our proprietary INA® technologies. HydrolEasy™ probes provide increased signal-to-noise ratio and increased specificity and sensitivity toward the target sequence compared to standard TaqMan hydrolysis probes.
Standard DNA primers and TaqMan probe
Standard DNA primers and HydrolEasy™ probe
Real-time PCR amplification plots of SARS-CoV-2 assay with standard DNA primers and TaqMan probe (left) and standard DNA primers and HydrolEasy™ probe (right).