PlentiPlex® is a series of ready-to-use PCR-based assays for convenient and accurate analysis of DNA including genotyping, microsatellite instability and somatic mutation detection.
Common for PlentiPlex® assays is that they work on open instruments and rely on PentaBase's INA® platform technologies; SuPrimers™, EasyBeacons™, HydrolEasy™ probes and BaseBlockers™, to assure ease-of-use, robustness and optimal analysis performance.
PlentiPlex® assays include PlentiPlex® genotyping assays for fast, reliable and unambiqious analysis of single nucleotide polymorphisms. Use PlentiPlex® MSI for easy and fully multiplexed determination of microsatellite instability, and PlentiPlex® Mastocytosis for highly sensitive and selective detection of somatic mutations in hematopoetic cells.
The PlentiPlex® genotyping assays offer extremely easy, fast and multiplexed SNP determination on standard real-time PCR instrumentation. We have a range of SNP assays for genes involved in tolerance, diet and exercise in stock, but are also developing new assays on demand.
PlentiPlex® MSI assays are intended for in vitro diagnosis of impaired DNA mismatch repair system (MMR) affecting prognosis and selection of treatment in cancer patients. PlentiPlex® MSI assays offer two different multiplexed MSI assays for the length analysis of five micro-satellite loci. The mono- and di-nucleotide panel recommended by the Bethesda guidelines and, the Pentaplex mono-nucleotide panel introduced by Hamelin, enabling evaluation of MMR status on DNA sequencer instruments.
Micro-satellites are genetic motifs consisting of short (one to six bases) nucleotide sequences repeated up to 100 times. During replication, these sequences are susceptible to errors, deletions and insertions, normally corrected by the DNA mismatch repair system. Uncorrected micro-satellite errors are commonly referred to as micro-satellite instability, MSI.
Two examples of micro satelitte instability samples. The tumor tissues (bottom) is compared to non-tumor tissues (top). Shown is capillary gel migration charts with MSI peaks marked with red brackets.
The PlentiPlex® Mastocytosis assay combines the high selectivity of SensiScreen® FFPE assays with the extreme sensitivity of SensiScreen® Liquid, enabling detection of single copy mutants in concentrated DNA samples.
Mastocytosis is the common term for a heterogenous group of diseases characterized by abnormal accumulation and growth of neoplastic mast cells in tissue and organs.
KIT D816V mutation is almost always present in Mastocytosis patients and this somatic alteration is among the WHO criteria for diagnosis of mastocytosis.