Microsatellites are genetic motifs consisting of short nucleotide sequences repeated up to 100 times and comprising approximately 3% of the human genome. During replication, these regions are particularly susceptible to mutations, normally corrected by the DNA mismatch repair (MMR) system. Microsatellite instability (MSI) refers to the accumulation of uncorrected microsatellite errors which are used for detection of MMR deficiency implicated in diagnosis and treatment of human cancers.
A Revolutionizing Method for Evaluation of Microsatellite Instability
MicroSight® MSI is a new, simple and fast way of evaluating microsatellite instability (MSI) status of human DNA. The analysis is based on PentaBase’s proprietary INA® technology that enables fast and easy detection of microsatellite instability status by real-time PCR and high-resolution melt (HRM) analysis. MicroSight® MSI requires very little hands-on time and enables evaluation of patient microsatellite status of purified human genomic DNA in less than one and a half hours for fast treatment guidance of cancer patients.
MicroSight® MSI workflow. Only three fast and simple steps are needed for the analysis of microsatellite instability with MicroSight® MSI. The method is based on high-resolution melt analysis and gives a clear and objective evaluation of microsatellite status.
Evaluation of well-established MSI markers
MicroSight® MSI PentaBase Panel evaluates instability of the well-established MSI markers BAT25, BAT25, NR22, NR24 and MONO27 allowing for easy conversion from current methods based on capillary electrophoresis.
Analysis of paired and unpaired tumour samples
MicroSight® MSI can be used for analysis of both paired and unpaired tumour samples. Evaluation of unpaired tumour samples is done by comparison to a universal reference assay supplied by PentaBase (Strip HR). Up to three unpaired tumour samples can be analysed in each real-time PCR run.
Evaluation of microsatellite instability by MicroSight® MSI requires special software for analysis of HRM data which is currently supported by PentaBase's BasecCycler™ as well as the MyGo Pro (IT-IS Life Science Ltd.) real-time PCR instruments.