Skip to content
LoginCreate Account
MENUMENU
  • Contact
  • Technical Support
  • Ordering
  • Find your local distributor
  • Become a distributor
  • Resources
  • Policies
  • 🛒 CART
WOOCS 2.2.4
PentaBase
MENUMENU
  • BOOK TEST
  • COVID-19
    • BOOK COVID-19
    • COVID-19 for Companies
    • CoviDetect™ Assay
    • CoviDetect™ FAST Assay
    • SARS-CoV-2 Oligos
    • SARS-CoV-2 Mutations
  • Products
    • Diagnostics
    • Equipment
    • Custom Oligos
    • Consumables
    • Reagents
    • Sequencing
  • Ordering
    • How to order
    • General Terms & Conditions
  • Services
    • SNP Genotyping Services
    • Real-Time PCR Assay Development
  • Technology
    • NGS Adapters
    • BaseBlockers™
    • (Su)Primers™
    • Fluorescent Probes
  • About Us
    • News
    • Mission and Visions
    • Meet the team
    • Advisory Board
    • Quality Management System
    • PentaBase in the Press

Frontpage - Molecular Diagnostics - Cancer Mutations - SensiScreen® Liquid

Our Products

  • Cancer Mutations
    • SensiScreen® Cancer IVD Assays
    • PlentiPlex™ MYD88
  • Microsatellite Instability
    • MicroSight® MSI Assays
    • PlentiPlex™ MSI
  • Mastocytosis
    • PlentiPlex™ Mastocytosis
  • Sequencing
    • NGS Adapters
  • Reagents
    • cDNA Supreme
  • Consumables
    • Vials and tubes
    • Pipette tips
  • Equipment
    • Real-time PCR Instruments
    • DNA Purification Instrument
  • COVID-19 BOOKING
    • Quick antibody test (IgM-IgA/IgG) in Aarhus
    • Antibody test (IgM) in Herlev
    • Quick antibody test (IgM-IgA/IgG) in Esbjerg
    • Antibody test (IgM) in Odense
    • COVID-19 RT-PCR test in Aarhus
    • COVID-19 RT-PCR test in Herlev
    • COVID-19 RT-PCR test in Odense
    • COVID-19 RT-PCR test in Esbjerg

Our Technologies

  • NGS Adapters
  • BaseBlockers™
  • (Su)Primers™
  • Fluorescent Probes

SensiScreen® Liquid

Cancer mutation detection in liquid biopsies

SensiScreen® Liquid assays are designed for early detection and monitoring of somatic mutations in liquid biopsies using standard equipment. With SensiScreen® Liquid, it is possible to monitor changing levels of cancer mutations and detect newly developed mutations in cell-free DNA (cfDNA) shed into the blood or other liquid biopsies. In this way, SensiScreen® Liquid can support the selection of the most appropriate personalized treatment in a timely manner.

Sensiscreen liquid

SensiScreen® Liquid are real-time PCR-based assays that combine high sensitivity with ease-of-use and are designed to work on standard real-time PCR equipment. Sensitive detection of somatic mutations by SensiScreen® is achieved with PentaBase’s novel and selective INA® technologies including the use of BaseBlockers™ that suppress false positive signals from wild type templates. Thus, SensiScreen® Liquid assays are able to detect down to a single copy of mutated DNA in a background of wild type DNA.

Format

SensiScreen® assays are supplied as either “Ready-to-use” or as “Dispense Ready” mixes. Ready-to-use assays are pre-dispensed in PCR strips and require only addition of gDNA for somatic mutation testing. Dispense-ready mixes include oligonucleotides and master mix in separate tubes ready to be dispensed and added gDNA. The technologies are applicable on standard real-time equipment using standard procedures and results can be ready in less than three hours from receiving a sample (including automated DNA extraction).

Covered Mutations

SensiScreen® Liquid is a cancer detection kit for liquid biopsies. SensiScreen® Liquid assays cover the most common clinically relevant cancer mutations including those in BRAF, EGFR, KRAS and NRAS genes.Explore the mutations below or refer to the Instructions For Use for more details. Please note that all SensiScreen® assays are also available as an FFPE version for detection in solid biopsies.

Visit our SensiScreen® product page for prices and more information.

Liquid Mutations

BRAF V600
SensiScreen® BRAF V600 Liquid assays identify 4 mutations in BRAF codon 600.
Intended use SensiScreen® BRAF V600 Liquid assays are real-time PCR-based assays intended for identification of the mutations V600D/E/K/R in the BRAF gene. SensiScreen® BRAF V600 Liquid assays are CE-IVD marked in accordance with EU Directive 98/79/EC on Medical Equipment for in vitro diagnostics.
Detection of BRAF mutations by SensiScreen® Liquid include the following assays:
AssayStatus
BRAF V600D/E/K/R MultiplexCE-IVD
BRAF V600D/E/K/R SimplexCE-IVD
BRAF V600E SimplexCE-IVD
EGFR Exon 18, 19, 20, 21
SensiScreen® EGFR Liquid assays identify 63 mutations in EGFR exon 18, 19, 20 and 21.
Intended use: SensiScreen® EGFR Liquid assays are real-time PCR-based assays intended for identification of mutations in exons 18, 19, 20 and 21 of the EGFR gene. SensiScreen® EGFR Exon 19 Deletions, T790M and L858R Liquid assays are CE-IVD marked in accordance with EU Directive 98/79/EC on Medical Equipment for in vitro diagnostics. The remaining SensiScreen® EGFR Liquid assays are intended for research applications only.
Detection of EGFR mutations by SensiScreen® Liquid include the following assays:
AssayStatus
EGFR Exon 18+19+20+21RUO
EGFR G719A/C/SRUO
EGFR Exon 19 Deletions (35 deletions)CE-IVD
EGFR S768IRUO
EGFR T790MCE-IVD
EGFR Exon 20 Insertions (22 insertions)RUO
EGFR L858RCE-IVD
EGFR L861QRUO
EGFR Exon 19 Deletions+T790M+L858RCE-IVD
KRAS Exon 2, 3, 4
SensiScreen® KRAS Liquid assays identify 23 mutations in KRAS exon 2, 3 and 4.
Intended use SensiScreen® KRAS Liquid assays are real-time PCR-based assays intended for identification of mutations in codons 12, 13, 59, 61, 117 and 146 of the KRAS gene. SensiScreen® KRAS Liquid assays are intended for research applications only.
Detection of KRAS mutations by SensiScreen® Liquid include the following assays:
AssayStatus
KRAS Exon 2+3+4 MultiplexRUO
KRAS Exon 2 Multiplex
G12A/C/D/R/S/V
G13D
RUO
KRAS Exon 3 Multiplex
Q61H1/H2/E/K/L/R
A59G/T
RUO
KRAS Exon 4 Multiplex
K117N1/N2
A146P/T/V
RUO
KRAS Exon 2 Simplex
G12A/C/D/R/S/V
G13D
RUO
KRAS Exon 3 Simplex
Q61H1/H2/E/K/L/R
A59G/T
RUO
KRAS Exon 4 Simplex
K117N1/N2
A146P/T/V
RUO
KIT D816V
The SensiScreen® KIT Liquid assay kit identifies the aspartic acid (D) to valine mutation in codon 816 of the KIT gene (D816V).
Intended use: The SensiScreen® KIT Liquid assay is a real-time PCR-based assay intended for identification of the KIT D816V mutation. The SensiScreen® KIT Liquid assay is intended for research appplications only.
Detection of KIT mutations by SensiScreen® Liquid includes the following assay:
AssayStatus
KIT D816VRUO
NRAS Exon 2, 3 and 4
SensiScreen® NRAS Liquid assays identify 24 mutations in NRAS exon 2, 3 and 4.
Intended use SensiScreen® NRAS Liquid assays are real-time PCR-based assays intended for identification of mutations in codons 12, 13, 59, 61, 117 and 146 of the NRAS gene. SensiScreen® NRAS Liquid assays are intended for research applications only.
Detection of NRAS mutations by SensiScreen® Liquid include the following assays:
AssayStatus
NRAS Exon 2+3+4 MultiplexRUO
NRAS Exon 2 Multiplex
G12A/C/D/R/S/V
G13A/C/D/R/S/V
RUO
NRAS Exon 3 Multiplex
Q61H1/H2/K/L/R
A59D/T
RUO
NRAS Exon 4 Multiplex
K117N1/N2
A146P/T/V
RUO
NRAS Exon 2 Simplex
G12A/C/D/R/S/V
G13A/C/D/R/S/V
RUO
NRAS Exon 3 Simplex
Q61H1/H2/K/L/R
A59D/T
RUO
NRAS Exon 4 Simplex
K117N1/N2
A146P/T/V
RUO
PiK3CA H1047
SensiScreen® PIK3CA H1047 Liquid assays identify three mutations in codon 1047.
Intended use SensiScreen® PIK3CA H1047 Liquid assays are real-time PCR-based assays intended for identification of the mutations H1047L/R/Y in the PIK3CA gene. SensiScreen® PIK3CA H1047 FFPE assays are intended for research applications only.
Detection of PIK3CA mutations by SensiScreen® Liquid include the following assays:
AssayStatus
PIK3CA H1047L/R/Y MultiplexRUO
PIK3CA H1047L/R/Y SimplexRUO

Key features and benefits of SensiScreen® for liquid biopsies


  • Liquid sample analysis: Liquid version for analysis of liquid biopsies containing small amounts of DNA.
  • High Sensitivity: SensiScreen® Liquid detects down to one copy of mutated DNA in a wild type DNA background of up to 5 ng.
  • Easy and fast: Easy to setup (hands-on-time <2 min/sample) and takes less than three hours from sample to result including DNA extraction.
  • State of the art: Based on PentaBase’s proprietary INA® technology.
  • Proven: SensiScreen® CE IVD assays are clinically validated in Switzerland and Denmark, and are certified in accordance to the EU guidelines 98/79/EC Medical equipment for in vitro diagnostics.
  • Simplex and multiplex: Supplied in multiplex configurations for mutation screening and simplex configurations for genotyping and monitoring.
  • Open qPCR platform designs: Designed to work on standard real-time PCR instruments and validated on commonly used platforms:.
Rotor-Gene Q (Qiagen) CFX96™ (Bio-Rad)
MX3000P/3005P (Agilent) ABI 7500/7900HT/QuantStudio™ 12K Flex (Applied Biosystems)
LightCycler® 96 + 480 (Roche) MyGo Pro (IT-IS Life Science)
Mic (Bio Molecular Systems)

Experimental Data Example

The figures below illustrate a SensiScreen® Liquid assay detecting one copy of mutated DNA. Real-time PCR amplification of 12 replications of patient DNA Heterozygous for an EGFR Exon 19 Deletion diluted to approximately 3 copies per well was performed.

  • Contact PentaBase
    • General Inquiries
    • How to Order
    • Technical Support
    • Request Demo Kit

Resources

Cancer Mutation Technologies

Product information

SensiScreen® Overview
SensiScreen® Brochure

Publications

SensiScreen® KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations

Instructions for use

FFPE Assays
Liquid Assays

Quick Guides

FFPE ready-to-use
FFPE dispense ready
Liquid ready-to-use
Liquid dispense ready

CONTACT US

  • Email: moc.esabatnep@ofni
  • Phone: +45 36 96 94 96
  • CVR: 30 19 78 52

VISIT US

PentaBase ApS
Petersmindevej 1A
DK-5000 Odense C
Denmark

KEEP IN TOUCH

FOLLOW US

This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Cookie settingsACCEPT
Privacy & Cookies Policy

Privacy Overview

This website uses cookies to improve your experience while you navigate through the website. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may have an effect on your browsing experience.
Necessary
Always Enabled

Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.

Non-necessary

Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.

×
Item Added to your Cart!
There are no products
Continue Shopping