We convert your TaqMan^® or Molecular Beacon designs into the corresponding HydrolEasy^® probe or EasyBeacon™ respectively. The service is only available in combination with a confirmed order of a HydrolEasy^® probe or an EasyBeacon™. We also convert standard DNA primers into SuPrimers™.

You need to supply:

• The sequence of your current probe and/or primer design or the sequences found by using your desired primer/ probe design programme (a free online design programme can be found here: Primer3).
• Fluorophore and quencher of your choice: e.g. FAM and TAMRA
• Please also mention other demands like reading temperature.
  
  

• A quote for purchasing the primers/probes comprising PentaBase's technologies followed by
• The ordered Primers/probes comprising our HyNA technology, delivered in nice storage plastic boxes.
• Synthesis reports and other documentation 

Price: Free of Charge (primers and probes have to be purchased)

Order online or send an email

PentaBase offers it's expert knowledge and experience in designing primers and probes for your target gene.

You need to supply:

• A gene sequence or a reference number to NCBI gene database.
• Explain what type of assay you need (expression, SNP, etc.) and the origin of the genetic material.
• Indicate if you prefer DNA primers or SuPrimers™ and what probe type (EasyBeacons™ or HydrolEasy^® probes) that you prefer (or ask for advice).

• Primer/probe sequences that are meeting your specifications, designed following the golden standards of rules and methods.
• The service includes follow up support using our online support module.

Do you have challenging targets like finding consensus sequences or discriminating between several genotypes? Are you designing multiplex assays? Let PentaBase use their expert knowledge and experience in helping you with the assay design.

You need to supply:

• The relevant electronic sequence information (the alignment or different target sequences) or a reference for that.
• Special considerations that has to be taken.

• An assay designed after the golden standards of assay design, using PentaBase's technologies.
• A report explaining the designed assay in details.
• Follow-up support is included through our online chat support, email or telephone.
• Improvements in design if needed (re-design for free, however primers and probes has to be paid for unless they are not working due to mistakes made by PentaBase).

Do you have a Real-time PCR assay, but you are not satisfied with it's performance? Are you developing a new assay, but having problems getting it sensitive or specific enough to meet you criterias? In other words would you like a helping hand with optimisation of your real-time PCR assays? Would you like to develop your own duo-plex (bi-plex) or multiplex PCR? PentaBase offers a very convenient solution.

You need to supply:

• Inactivated template used for optimizing the assay and comparing to your existing assay (control DNA, enough for min. 100 reactions).
• The scope of the optimization (increase sensitivity, specificity, signal-to-noise ratio, develop a new assay that are able to ..., and so on)
• If we should help you improve an existing assay please provide sequences of your present assay and a sample (enough for min. 25 assays, preferably with primers and probes provided separately).

• An optimized assay that is guaranteed to live up to your expectations (you will be presented with data before you have to accept the end-point of the assay).
• A report describing the assay in details.
• Comparative results obtained in the laboratories of PentaBase.
• 5 nmol of the primers and/or probes for the assay.
• After sales support using our online chat function, email or telephone

It happens again and again. When customers have tried PentaBase's technologies, they want to change their whole portfolio using our technology.  We have therefore made it possible for customers to hire one of PentaBase's experienced employees to dedicate their time exclusively to the customer's project. All employes will be guided by the executive scientific management team of PentaBase.

By hiring "your own" PentaBase employee, you can make sure that all optimization and developments are done according to your specifications. You can for example dictate which master mix and conditions your assays should be optimised for, if the assays should be tested together with other assays, keep optimizing the assay untill it meets your satisfaction, etc. So basically it would work like your own employee, however with the experience to make real-time PCR optimisation and handling PentaBase's superior technologies.

• Project details includinge aim of project
• Conditions that the assay should be carried out under
• You also need to supply special reagents if needed like: Inactivated template used for optimizing the assay, master mix, reference assay, control assays etc.
• Response to the results that you are given
  

• A PentaBase employee that will work exclusively on your project, optimizing your assay according to your instructions and that is under the guidance of PentaBase's executive scientists.
• A monthly report describing progress of your assay.
• A detailed report including data, results and conclusions of the assay(s) in details.
• 5 nmol of the primers and/or probes for the assays.
• Other assistance that might be needed for example in relation to IP protection of your assays
  
• After sales support using our online chat function, email or telephone