November 18, 2008
SERVICE examples
  

Service      
Sequencing from only €2.99!
PentaBase now offers very price competitive sequencing. To give you most value for the money, we carry out our sequencing in Asia.

We introduce this new service by giving you an irresistible offer!

Sequencing purified DNA using standard primers? €2,99 per sample.

Sequencing clones? No problem we will grow the clones and extract DNA. Only €3,99 including standard primers.

Sequencing reactions are performed on an ABI 3730xl. Maximum sequencing length on this machine is 700-900 nucleotides. Prices are only valid for a limited period. Prices are excl. VAT and shipping.

Please contact us at support@pentabase.com for further information and to receive a no-obligation quote, or use our online chat function to get instant answers on your questions.


Large Sequencing projects—no problem!

No project seems to big for us. We have access to the largest sequencing facilities in Asia:

21 ABI 3730XL - Applied Biosystems

17 Solexa Genome Analyzers - Illumina

3 454 / GS FLX - Roche

3 SOLID™ Systems - Applied Biosystems

Please contact us for quotes and discussions on larger projects.

info@pentabase.com

  

five different service levels
Below is described the 5 different service levels PentaBase offers. Please browse through the page and the examples in the sub-menus.

We hope to help you with your assay development and optimisation
  

Level 1: improve existing assay - change of technology

service level 1 thumb.jpgWe convert your TaqMan® or Molecular Beacon designs into the corresponding HydrolEasy® probe or EasyBeacon™ respectively. The service is only available in combination with a confirmed order of a HydrolEasy® probe or an EasyBeacon™. We also convert standard DNA primers into SuPrimers™.

You need to supply:

  • The sequence of your current probe and/or primer design or the sequences found by using your desired primer/ probe design programme (a free online design programme can be found here: Primer3).
  • Fluorophore and quencher of your choice: e.g. FAM and TAMRA
  • Please also mention other demands like reading temperature.

You will receive:
  • A quote for purchasing the primers/probes comprising PentaBase's technologies followed by
  • The ordered Primers/probes comprising our HyNA technology, delivered in nice storage plastic boxes.
  • Synthesis reports and other documentation 

Price: Free of Charge (primers and probes have to be purchased)

Order online or send an email

  

Level 2: Primer & probe design - one sequence

service level 2 thumb.jpgPentaBase offers it's expert knowledge and experience in designing primers and probes for your target gene.

You need to supply:

  • A gene sequence or a reference number to NCBI gene database.
  • Explain what type of assay you need (expression, SNP, etc.) and the origin of the genetic material.
  • Indicate if you prefer DNA primers or SuPrimers™ and what probe type (EasyBeacons™ or HydrolEasy® probes) that you prefer (or ask for advice).
You will receive:
  • Primer/probe sequences that are meeting your specifications, designed following the golden standards of rules and methods.
  • The service includes follow up support using our online support module.
Price: Start € 325

Send an email to inquire
     

Level 3: Alignments and multiplex assays

service level 3 thumb.jpgDo you have challenging targets like finding consensus sequences or discriminating between several genotypes? Are you designing multiplex assays? Let PentaBase use their expert knowledge and experience in helping you with the assay design.

You need to supply:

  • The relevant electronic sequence information (the alignment or different target sequences) or a reference for that.
  • Special considerations that has to be taken.
You will receive:
  • An assay designed after the golden standards of assay design, using PentaBase's technologies.
  • A report explaining the designed assay in details.
  • Follow-up support is included through our online chat support, email or telephone.
  • Improvements in design if needed (re-design for free, however primers and probes has to be paid for unless they are not working due to mistakes made by PentaBase).

Price: Starts at € 750

Send an email to inquire
     

Level 4: Assay optimization - satisfaction guaranteed

service level 4 thumb.jpgDo you have a Real-time PCR assay, but you are not satisfied with it's performance? Are you developing a new assay, but having problems getting it sensitive or specific enough to meet you criterias? In other words would you like a helping hand with optimisation of your real-time PCR assays? Would you like to develop your own duo-plex (bi-plex) or multiplex PCR? PentaBase offers a very convenient solution.

You need to supply:

  • Inactivated template used for optimizing the assay and comparing to your existing assay (control DNA, enough for min. 100 reactions).
  • The scope of the optimization (increase sensitivity, specificity, signal-to-noise ratio, develop a new assay that are able to ..., and so on)
  • If we should help you improve an existing assay please provide sequences of your present assay and a sample (enough for min. 25 assays, preferably with primers and probes provided separately).

You will receive:
  • An optimized assay that is guaranteed to live up to your expectations (you will be presented with data before you have to accept the end-point of the assay).
  • A report describing the assay in details.
  • Comparative results obtained in the laboratories of PentaBase.
  • 5 nmol of the primers and/or probes for the assay.
  • After sales support using our online chat function, email or telephone

Price: Starts at € 1,000


Send an email to inquire
     

Level 5: your external employee
service level 5 thumb.jpgIt happens again and again. When customers have tried PentaBase's technologies, they want to change their whole portfolio using our technology.  We have therefore made it possible for customers to hire one of PentaBase's experienced employees to dedicate their time exclusively to the customer's project. All employes will be guided by the executive scientific management team of PentaBase.

By hiring "your own" PentaBase employee, you can make sure that all optimization and developments are done according to your specifications. You can for example dictate which master mix and conditions your assays should be optimised for, if the assays should be tested together with other assays, keep optimizing the assay untill it meets your satisfaction, etc. So basically it would work like your own employee, however with the experience to make real-time PCR optimisation and handling PentaBase's superior technologies.

You need to supply:
  • Project details includinge aim of project
  • Conditions that the assay should be carried out under
  • You also need to supply special reagents if needed like: Inactivated template used for optimizing the assay, master mix, reference assay, control assays etc.
  • Response to the results that you are given

You will receive:
  • A PentaBase employee that will work exclusively on your project, optimizing your assay according to your instructions and that is under the guidance of PentaBase's executive scientists.
  • A monthly report describing progress of your assay.
  • A detailed report including data, results and conclusions of the assay(s) in details.
  • 5 nmol of the primers and/or probes for the assays.
  • Other assistance that might be needed for example in relation to IP protection of your assays
  • After sales support using our online chat function, email or telephone

Price: Starting from only €435 per day, depending on length of contract.
Please inquire for your particular project.

Send an email to inquire

  

General notes
PentaBase will treat all information received from customers as confidential. However as PentaBase are developing diagnostic kits and research use only kits, we retains the right to refuse a project if there are any conflict of interest. PentaBase will send an order confirmation before starting any service, so that the customer is fully aware of the price and other issues. Please be aware that positions of HyNA modifications will not be shown in sequence.

Any purchase from product purchased from PentaBase comes with a limited license for research use only. It is not allowed to on sell products or services including the use of PentaBase products. For commercial applications of PentaBase's products as in, for example, production of a product for sale in the research products market, offering a service, information or data, resale of a PentaBase product or a component or derivative of the product, a fee/royalty-based license will be necessary. For more information on licensing please contact Ulf Bech Christensen

TaqMan® is registered Trade Mark of the company Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA
     

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